Journal: Human cell

Article Title: Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes.

doi: 10.1007/s13577-023-00918-2

Figure Lengend Snippet: Fig. 6 A model for the mechanism of osteogenic differentiation of periodontal stem cells regulated by psoralen

Article Snippet: 100 μL cell suspension was added into centrifuge tubes, then mouse anti-human antibodies CD34 (E-AB-F1143C), CD45 (E-AB-F1137C), CD105 (E-AB-F1143D), CD73 (E-AB-F1242D), and CD90 (E-AB-F1167D) (Elabscience, Wuhan, China) (1:50) were added, respectively, PBS was added as the control group, and all cells were incubated at room temperature for 20 min in the dark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model

doi: 10.3390/ijms23063310

Figure Lengend Snippet: Flow cytometry analysis of isolated porcine Wharton´s jelly mesenchymal stem cells (pWJCs).

Article Snippet: 34 , 0.38% , Biorbyt (Cambridge, UK) , orb247244.

Techniques: Flow Cytometry, Isolation, Marker

Journal: Molecular Medicine Reports

Article Title: MRI tracing of ultrasmall superparamagnetic iron oxide nanoparticle-labeled endothelial progenitor cells for repairing atherosclerotic vessels in rabbits

doi: 10.3892/mmr.2020.11431

Figure Lengend Snippet: Morphological characteristics of rabbit EPCs. (A) Cells changed from a globe-like shape to a thin structure, with acquiring a fusiform pebble-like shape at day 10. Magnification, ×20. (B) EPCs formed a capillary-like structure after 48 h on Matrigel. Magnification, ×20. (C-H) Expression levels in EPCs of the endothelial cells surface markers (C and E) CD133, (D and G) CD34, (F) CD31 and (H) VEGFR2 were 98.1, 3.4, 83.4 and 52.1%, respectively. EPCs, endothelial progenitor cells; FACS, fluorescence-activated cell sorting; VEGFR-2, vascular endothelial growth factor receptor 2.

Article Snippet: Cells were washed with PBS and incubated with FITC-CD34 (1:100; cat. no. bs-0646R-FITC; BIOSS), PE-CD133 (1:100; cat. no. bs-0395R-PE; Beijing Biosynthesis Biotechnology, Co., Ltd.), FITC-CD31 (1:100; cat. no. bs-0195R-FITC; Beijing Biosynthesis Biotechnology, Co., Ltd.) and PE-VEGF receptor 2 (1:100; cat. no. bs-10412R-PE; Beijing Biosynthesis Biotechnology, Co., Ltd.) primary antibodies at 4°C for 20 min. Rabbit auricular whole blood (1 ml) was collected and mixed with 2 ml erythrocyte lysate (BD Biosciences).

Techniques: Expressing, Fluorescence, FACS

Journal: Molecular Medicine Reports

Article Title: MRI tracing of ultrasmall superparamagnetic iron oxide nanoparticle-labeled endothelial progenitor cells for repairing atherosclerotic vessels in rabbits

doi: 10.3892/mmr.2020.11431

Figure Lengend Snippet: MRI of rabbits treated with USPION-labeled EPCs and control animals. (A) MRI of rabbits treated with USPION-labeled EPCs showed no significant changes in aortic vessels at (A) 1 day (B) 7 days and (C) 16 days after infusion. (D) MRI of rabbits in the control group. (E) Injured vessels were stained with Prussian blue 1 day after transplantation of USPION-labeled EPCs. Magnification, ×40. (F) Lesion vessels were stained with Prussian blue 7 days after the transplantation of USPION-labeled EPCs. Magnification, ×40. (G) Prussian blue staining of rabbit blood vessels in the control group. Magnification, ×40. EPCs, endothelial progenitor cells; USPION, ultrasmall superparamagnetic iron oxide nanoparticle.

Article Snippet: Cells were washed with PBS and incubated with FITC-CD34 (1:100; cat. no. bs-0646R-FITC; BIOSS), PE-CD133 (1:100; cat. no. bs-0395R-PE; Beijing Biosynthesis Biotechnology, Co., Ltd.), FITC-CD31 (1:100; cat. no. bs-0195R-FITC; Beijing Biosynthesis Biotechnology, Co., Ltd.) and PE-VEGF receptor 2 (1:100; cat. no. bs-10412R-PE; Beijing Biosynthesis Biotechnology, Co., Ltd.) primary antibodies at 4°C for 20 min. Rabbit auricular whole blood (1 ml) was collected and mixed with 2 ml erythrocyte lysate (BD Biosciences).

Techniques: Labeling, Staining, Transplantation Assay